https://www.nature.com/articles/nm.3985 6park.com
https://www.nature.com/articles/nm.3985.pdf 6park.com
2015年发表的文章，还怎么抵赖呢？ 6park.com
五毛看不懂英文的，去问问你们主子的主子的主子，看是不是有这个事情 6park.com
A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence
                            Vineet D Menachery, Boyd L Yount Jr, Kari Debbink, Sudhakar Agnihothram, Lisa E Gralinski, Jessica A Plante, Rachel L Graham, Trevor Scobey, Xing-Yi Ge, Eric F Donaldson, Scott H Randell, Antonio Lanzavecchia, Wayne A Marasco, Zhengli-Li Shi & Ralph S Baric 
                            
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    Nature Medicine 6park.com
                                volume 21, pages1508–1513(2015)Cite this article
                            

                            
    
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        A Corrigendum to this article was published on 06 April 2016
        
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                        This article has been updated
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Abstract
The
 emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) 
and Middle East respiratory syndrome (MERS)-CoV underscores the threat 
of cross-species transmission events leading to outbreaks in humans. 
Here we examine the disease potential of a SARS-like virus, SHC014-CoV, 
which is currently circulating in Chinese horseshoe bat populations1. Using the SARS-CoV reverse genetics system2,
 we generated and characterized a chimeric virus expressing the spike of
 bat coronavirus SHC014 in a mouse-adapted SARS-CoV backbone. The 
results indicate that group 2b viruses encoding the SHC014 spike in a 
wild-type backbone can efficiently use multiple orthologs of the SARS 
receptor human angiotensin converting enzyme II (ACE2), replicate 
efficiently in primary human airway cells and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo
 experiments demonstrate replication of the chimeric virus in mouse lung
 with notable pathogenesis. Evaluation of available SARS-based 
immune-therapeutic and prophylactic modalities revealed poor efficacy; 
both monoclonal antibody and vaccine approaches failed to neutralize and
 protect from infection with CoVs using the novel spike protein. On the 
basis of these findings, we synthetically re-derived an infectious 
full-length SHC014 recombinant virus and demonstrate robust viral 
replication both in vitro and in vivo. Our work suggests a potential risk of SARS-CoV re-emergence from viruses currently circulating in bat populations.

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