关于冠状病毒的来源,2015年自然杂志的那篇论文分析
https://www.nature.com/articles/nm.3985 6park.com论文是中美瑞共同完成的,15名作者中12人来自美国,1人来自瑞士,2人来自中国Vineet D Menachery, Boyd L Yount Jr, Kari Debbink, Sudhakar Agnihothram, Lisa E Gralinski, Jessica A Plante, Rachel L Graham, Trevor Scobey, Xing-Yi Ge, Eric F Donaldson, Scott H Randell, Antonio Lanzavecchia, Wayne A Marasco, Zhengli-Li Shi& Ralph S Baric 6park.com中国科学院武汉病毒学研究所特殊病原体与生物安全重点实验室的参与2人Xing-Yi Ge & Zhengli-Li Shi 6park.com中国参与的2人贡献是 X.-Y.G.进行假分型试验,Z.-L.S.提供SHC014棘突序列和质粒ContributionsV.D.M. designed, coordinated and performed experiments, completed analysis and wrote the manuscript. B.L.Y. designed the infectious clone and recovered chimeric viruses; S.A. completed neutralization assays; L.E.G. helped perform mouse experiments; T.S. and J.A.P. completed mouse experiments and plaque assays; X.-Y.G. performed pseudotyping experiments; K.D. generated structural figures and predictions; E.F.D. generated phylogenetic analysis; R.L.G. completed RNA analysis; S.H.R. provided primary HAE cultures; A.L. and W.A.M. provided critical monoclonal antibody reagents; and Z.-L.S. provided SHC014 spike sequences and plasmids. R.S.B. designed experiments and wrote manuscript.
Corresponding authors 什么是假分型实验,简单的说就输入步骤-病毒侵入细胞过程中,病毒的外壳,通过与细胞受体分子的相互作用Why pseudotype?Pseudotyped vectors have several experimental and clinicalapplications (Box 1). Firstly, the outer shell of a virus,through its interaction with cellular receptor molecules,plays a major role in determining the tropism of a virus,specifically at the entry step. Pseudotyping a viral vectorcan thereby provide it with an expanded ensemble oftarget cells or can restrict it to specific cells that are theobject of experimental or therapeutic interest. Secondly, apseudotyped vector can have an altered stability and/orinteraction with the host immune system that increases itsefficacy. One example is pseudotyped viral vectors that canbe produced and/or concentrated to higher transductiontiters than the viral vector with its native outer shell.Thirdly, comparison of transduction by a pseudotypedvector and by a vector bearing a native shell can be used toidentify processes that are peculiar to entry mediated bythe native proteins. Finally, the entry process of the virusfrom which the outer shell of the pseudotyped virus isderived can be illuminated. 6park.com什么是棘突序列和质粒--简单说就是 DNA材料 DNA spiking. ... DNA spiking can also refer to a spike control in PCR, which is when DNA is added to a sample that will provide some signal (e.g. a plasmid or some synthetic DNA with a specific known sequence) to a reaction, and seeing if the reaction will amplify. 6park.com en.wikipedia.org 6park.comA plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes. Often, the genes carried in plasmids provide bacteria with genetic advantages, such as antibiotic resistance. 6park.com
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